Effects of Nutrients and Growth Regulators on Seed Germination and Development of Juvenile Rhizome Proliferation of Gastrodia elata In Vitro

Two prevalent issues with Gastrodia elata growing in a natural wild environment are the necessary symbiotic seed germination Mycena spp. and long cycle of protocorms into mature rhizomes Armillaria mellea. This study explored most suitable nutrients to germinate seeds developmental stages propagated protocorm/juvenile (jrhs) through tissue culture approach. Adding humic acid, mashed potato, peptone, (indole-3-acetic acid) IAA 2 g/L Hyponex 7-6-19 basal medium accelerated an average length mm. The best for vitro propagation jrhs consisted 1 MS salts 1/2 NH4NO3 KNO3, mg/L BA, NAA, tryptone, glutamic chitosan, 10 apple puree, 20 100 mL/L coconut water, 1.5 A. mellea powder, at pH level 6. maximum number were 17.2 ± 0.8 mm 76.6, respectively, two subcultures. system started from G. can be employed as potential method propagate protocorm/jrhs cultivation indoor or outdoor cultures.